Genetic heterogeneity of tumor is closely related to its clonal evolution, phenotypic diversity and treatment resistance, and such heterogeneity has only been characterized at single-cell sub-chromosomal scale in liver cancer. Here we reconstructed the single-variant resolution clonal evolution in human liver cancer based on single-cell mutational profiles. The results indicated that key genetic events occurred early during tumorigenesis, and an early metastasis followed by independent evolution was observed in primary liver tumor and intrahepatic metastatic portal vein tumor thrombus. By parallel single-cell RNA-Seq, the transcriptomic phenotype of HCC was found to be related with genetic heterogeneity. For the first time we reconstructed the single-cell and single-variant clonal evolution in human liver cancer, and dissection of both genetic and phenotypic heterogeneity will facilitate better understanding of their relationship.
基金：
National Natural Science Foundation of China (81802806, 82073116,
81902561, 81761138045), China National Science and Technology Major Project
for Prevention and Treatment of Infectious Diseases (2017ZX10203207),
SJTU Interdisciplinary Program (YG2021QN80, ZH2018ZDA33), SJTU Scientific
and Technological Innovation Funds (2019TPA09), Natural Science Foundation
of Shanghai (19140902500, 20511101900, 20ZR1427200), and Key Laboratory
of Systems Biomedicine (Ministry of Education) Grant (KLSB2020QN-04).
第一作者机构：[1]Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[3]Fudan Univ, Huashan Hosp, Dept Gen Surg, Shanghai, Peoples R China[4]Fudan Univ, Canc Metastasis Inst, Shanghai, Peoples R China
推荐引用方式(GB/T 7714)：
Su Xianbin,Zhao Linan,Shi Yi,et al.Clonal evolution in liver cancer at single-cell and single-variant resolution[J].JOURNAL OF HEMATOLOGY & ONCOLOGY.2021,14(1):doi:10.1186/s13045-021-01036-y.
APA：
Su, Xianbin,Zhao, Linan,Shi, Yi,Zhang, Rui,Long, Qi...&Han, Ze-Guang.(2021).Clonal evolution in liver cancer at single-cell and single-variant resolution.JOURNAL OF HEMATOLOGY & ONCOLOGY,14,(1)
MLA：
Su, Xianbin,et al."Clonal evolution in liver cancer at single-cell and single-variant resolution".JOURNAL OF HEMATOLOGY & ONCOLOGY 14..1(2021)
医学