Proteomics Analyses Reveal Functional Differences between Exosomes of Mesenchymal Stem Cells Derived from The Umbilical Cord and Those Derived from The Adipose Tissue
Objective: We aimed to identify the differentially expressed proteins (DEPs) and functional differences between exosomes derived from mesenchymal stem cells (MSCs) derived from umbilical cord (UC) or adipose tissue (AD). Materials and Methods: In this experimental study, the UC and AD were isolated from healthy volunteers. Then, exosomes from UC-MSCs and AD-MSCs were isolated and characterized. Next, the protein compositions of the exosomes were examined via liquid chromatography tandem mass spectrometry (LC-MS/MS), followed by evaluation of the DEPs between UC-MSC and AD-MSC-derived exosomes. Finally, functional enrichment analysis was performed. Results: One hundred and ninety-eight key DEPs were identified, among which, albumin (ALB), alpha-II-spectrin (SPTAN1), and Ras-related C3 botulinum toxin substrate 2 (RAC2) were the three hub proteins resent at the highest levels in the protein-protein interaction network that was generated based on the shared DEPs. The DEPs were mainly enriched in gene ontology (GO) items associated with immunity, complement activation, and protein activation cascade regulation corresponding to 24 pathways, of which complement and coagulation cascades as well as platelet activation pathways were the most significant. Conclusion: The different functions of AD- and UC-MSC exosomes in clinical applications may be related to the differences in their immunomodulatory activities.
基金：
973 Project(2015CB931802 and 2017YFA0205301), Chinese
National Natural Scientific Fund (No.81327002 and
No.81803094), China Postdoctoral Science Foundation
(No. 2017M621486), Shanghai Jiaotong University
Medical Professionals Cross Fund (No. YG2016ZD10)
and Shanghai Engineering Research Center for Intelligent
diagnosis and treatment instrument (No.15DZ2252000).
第一作者机构：[1]Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Dept Instrument Sci & Engn, Inst Nano Biomed & Engn,Shanghai Engn Res Ctr Int, POB 20040,800, Shanghai, Peoples R China[2]Shanghai Jiao Tong Univ, Natl Ctr Translat Med, Collaborat Innovat Ctr Syst Biol, Shanghai, Peoples R China
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Dept Instrument Sci & Engn, Inst Nano Biomed & Engn,Shanghai Engn Res Ctr Int, POB 20040,800, Shanghai, Peoples R China[2]Shanghai Jiao Tong Univ, Natl Ctr Translat Med, Collaborat Innovat Ctr Syst Biol, Shanghai, Peoples R China[3]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Oncol, Sch Med, POB 200336,1111 Xian Xia Rd, Shanghai, Peoples R China[*1]200336, 1111 Xian Xia Road, Department of Oncology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, P.R. China[*2]20040, 800 Institute of Nano Biomedicine and Engineering, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instrument, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, China
推荐引用方式(GB/T 7714)：
Liu Bin,Qiao Guanglei,Cao Wen,et al.Proteomics Analyses Reveal Functional Differences between Exosomes of Mesenchymal Stem Cells Derived from The Umbilical Cord and Those Derived from The Adipose Tissue[J].CELL JOURNAL.2021,23(1):75-84.doi:10.22074/cellj.2021.6969.
APA：
Liu, Bin,Qiao, Guanglei,Cao, Wen,Li, Chenlu,Pan, Shaojun...&Cui, Daxiang.(2021).Proteomics Analyses Reveal Functional Differences between Exosomes of Mesenchymal Stem Cells Derived from The Umbilical Cord and Those Derived from The Adipose Tissue.CELL JOURNAL,23,(1)
MLA：
Liu, Bin,et al."Proteomics Analyses Reveal Functional Differences between Exosomes of Mesenchymal Stem Cells Derived from The Umbilical Cord and Those Derived from The Adipose Tissue".CELL JOURNAL 23..1(2021):75-84
