In this study, methionine sulfoxide (MetO) was identified as an active metabolite that suppresses adipogenesis after screening obese individuals versus the normal population. MetO suppressed the gene and protein expression of CCAAT/enhancer binding protein (C/EBP) alpha, adipocyte fatty acid binding protein 4 (FABP4), and the nuclear receptor peroxisome proliferator-activated receptor gamma (PPAR gamma) during human preadipocyte (HPA) differentiation. Adipogenesis decreased following MetO treatment; however, the preadipocyte number, proliferation, and apoptosis were unaffected. The activity of phosphorylated extracellular signal-related kinase (P-ERK) of the mitogen-activated protein kinase (MAPK) pathway was significantly inhibited in HPA after MetO treatment. Furthermore, treatment of preadipocytes with the selective P-ERK1/2 agonist Ro 67-7476 abolished the effect of MetO against adipogenesis suggesting that MetO function is dependent on the MAPK pathway. The mechanistic insights of adipogenesis suppression by MetO presented in this study shows its potential as an antiobesity drug.
基金：
Scientific Research Project of Wuxi Municipal Health Commission; National Key Research and Development Program of China; [2021YFC2701900]; [2021YFC2701903]
第一作者机构：[1]Yixing Peoples Hosp, Dept Pediat, Yixing, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Yixing Peoples Hosp, Dept Pediat, Yixing, Peoples R China[2]Shanghai Jiao Tong Univ, Tongren Hosp, Sch Med, Shanghai, Peoples R China[5]Shanghai Jiao Tong Univ, Tongren Hosp, Hongqiao Int Inst Med, Sch Med, 720 Xianxia Rd, Shanghai 200336, Peoples R China[6]Yixing Peoples Hosp, Dept Pediat, 75 Tongzhenguan Rd, Yixing 214200, Jiangsu, Peoples R China[*1]Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No.720 Xianxia Rd, Shanghai 200336, China.[*2]Department of Pediatrics, Yixing People's Hospital, No.75 Tongzhenguan Rd, Yixing, Jiangsu 214200, China
推荐引用方式(GB/T 7714)：
Qin Dani,Lei Yong,Xie Wen,et al.Methionine sulfoxide suppresses adipogenic differentiation by regulating the mitogen-activated protein kinase signaling pathway[J].CELL BIOLOGY INTERNATIONAL.2023,47(3):648-659.doi:10.1002/cbin.11964.
APA：
Qin, Dani,Lei, Yong,Xie, Wen,Zheng, Qiuju,Peng, Zhou...&Shen, Huiping.(2023).Methionine sulfoxide suppresses adipogenic differentiation by regulating the mitogen-activated protein kinase signaling pathway.CELL BIOLOGY INTERNATIONAL,47,(3)
MLA：
Qin, Dani,et al."Methionine sulfoxide suppresses adipogenic differentiation by regulating the mitogen-activated protein kinase signaling pathway".CELL BIOLOGY INTERNATIONAL 47..3(2023):648-659
