The present study aimed to investigate the in vitro effect of human adipose derived stem cells (hADSCs) and their secretions on viability of retinal cells and the axonal growth. After isolation, the rat retina was kept in containers covered with poly-D-lysine/laminin, after preparing the supernatant of hADSCs, the retina along with cells and hADSCs conditioned medium (CM) were co-cultured. The viabilitywasassesed by 3-[4,5dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay at days 1, 7 and 14, and axonal growth of retinal cellswasanalyzed by Image J software using Neurite J plug-in. The expression of PI3K/Akt pathway genes and its proteins were evaluated through real-timlyerase pochain reaction (PCR) and western blot, respectively. Anti-oxidant effects of hADSCs and their CM were measured by measuring theacellsretinlevel of thiobarbituric acid reactive substance (TBARS), ferric reducing ability of plasma (FRAP) and glutathione (GSH). Brain-derived eurotrophicnfactor (BDNF), neurotrophin-3 (NT-3) and NT-4 in the CM were measured by enzyme-linked immunosorbent assay (ELISA). The CM containedgh hlevels of BDNF, NT-3 and NT-4 compared to the control group. The expression of genes and proteins of the PI3K/Akt pathway alongevels with lof TBARS, FRAP and GSH was also increased in the group of hADSCs and their CM compared to the control group. The results of this studyowed sthat hADSCs can increase the axonal growth and survival of rat retinal cells through anti-inflammatory, anti-apoptotic, and paracrinestimulation, as well as through the PI3K/Akt pathway.