Alterations in circular RNA (circRNA) expression have a vital impact on the biological processes in cancer. Moreover, the expression pattern and roles of circRNAs in hepatocellular cancer (HCC) remain unclear. This study performed qRT-PCR to determine the regulated circRNAs in HCC tissues and cell lines. CCK8, 5-ethynyl-2'-deoxyuridine (EdU) assay, colony formation, cell cycle assay, apoptotic assay, transwell, and wound healing assay were conducted to assess the function of hsa_circ_0091570 or miR-1307 on cell proliferation, apoptosis, and migration in vitro. Mouse xenograft models were used to measure the functions of hsa_circ_0091570 in vivo. The decreased expression of hsa_circ_0091570 was associated with the pathological staging of HCC patients. Furthermore, inhibition of hsa_circ_0091570 promoted cell proliferation and migration, blocked cell apoptosis in HCC cell lines, and promoted tumor growth in the mouse xenograft model. RNA immunoprecipitation assay verified the interaction of hsa_circ0091570 and miR-1307. The miR-1307 inhibitor inhibited the function induced by hsa_circ_0091570 siRNA. Overall, hsa_circ_0091570 sponge miR-1307 as a ceRNA and regulate ISM1 expression by exerting functional roles in HCC.
第一作者机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Sch Med, Dept Gastroenterol, 1111 Xianxia Rd, Shanghai 2000336, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Sch Med, Dept Gastroenterol, 1111 Xianxia Rd, Shanghai 2000336, Peoples R China[2]Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Dept Intervent Oncol, 106 Pujian Rd, Shanghai 200127, Peoples R China[*1]Department of Interventional Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 106 Pujian Road, 200127, Shanghai, China.[*2]Department of Gastroenterology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, 2000336, Shanghai, China.
推荐引用方式(GB/T 7714)：
Wang Yu-Gang,Wang Tao,Ding Min,et al.hsa_circ_0091570 acts as a ceRNA to suppress hepatocellular cancer progression by sponging hsa-miR-1307[J].CANCER LETTERS.2019,460:128-138.doi:10.1016/j.canlet.2019.06.007.
APA：
Wang, Yu-Gang,Wang, Tao,Ding, Min,Xiang, Shi-Hao,Shi, Min&Zhai, Bo.(2019).hsa_circ_0091570 acts as a ceRNA to suppress hepatocellular cancer progression by sponging hsa-miR-1307.CANCER LETTERS,460,
MLA：
Wang, Yu-Gang,et al."hsa_circ_0091570 acts as a ceRNA to suppress hepatocellular cancer progression by sponging hsa-miR-1307".CANCER LETTERS 460.(2019):128-138
医学