Lymph node (LN) metastasis of gastric cancer (GC) is the strongest prognostic indicator for this disease; however, the majority of the LN metastasis profiles of GC remain unknown, which notably hinders the therapeutic efficacy in clinic. In the present study, an orthotopic model of human GC was established for investigation of time-dependent LN metastasis patterns in mice. Luciferase-expressing NCI-N87 human GC cells were injected into the subserosa of the gastric body, resulting in a tumor formation rate of 100%. LN metastasis at four different anatomical positions in the abdomen were characterized until week 10 after tumor cell injection using sensitive bioluminescence imaging and histopathological analyses. Skip LN metastases were observed at later stages (weeks 8-10) of the experiment. Metastases in other major organs, including liver, spleen and lung, were also examined. Characterization of this orthotopic GC model and metastasis patterns in LNs and major organs should aid in the preclinical GC research regarding the metastatic mechanism and drug development.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81401958, 81572998, 81773274]; Shanghai Municipal Commission of Health and Family Planning [ZK2015A25]; Shanghai Changning Commission of Health and Family Planning [YXMZK004]; Shanghai Municipal Science and Technology CommissionScience & Technology Commission of Shanghai Municipality (STCSM) [14ZR1431700, 16520710700]; 'Shu Guang' Program of Shanghai Education Development Foundation [16SG13]; Shanghai Municipal Education CommissionShanghai Municipal Education Commission (SHMEC) [16SG13]; Shanghai Collaborative Innovation Center for Translational Medicine [TM201731]
第一作者机构：[1]Shanghai Jiao Tong Univ, Shanghai Tongren Hosp, Sch Med, Dept Gen Surg, 1111 Xianxia Rd, Shanghai 200336, Peoples R China[2]Shanghai Jiao Tong Univ, Shanghai Tongren Hosp, Sch Med, Hongqiao Int Inst Med, 1111 Xianxia Rd, Shanghai 200336, Peoples R China[3]Shanghai Jiao Tong Univ, Dept Pharmacol & Chem Biol, Sch Med, Inst Med Sci, Shanghai 200025, Peoples R China[4]Yanbian Univ, Coll Med, Yanji 133002, Jilin, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Shanghai Tongren Hosp, Sch Med, Dept Gen Surg, 1111 Xianxia Rd, Shanghai 200336, Peoples R China[2]Shanghai Jiao Tong Univ, Shanghai Tongren Hosp, Sch Med, Hongqiao Int Inst Med, 1111 Xianxia Rd, Shanghai 200336, Peoples R China[3]Shanghai Jiao Tong Univ, Dept Pharmacol & Chem Biol, Sch Med, Inst Med Sci, Shanghai 200025, Peoples R China[*1]Department of General Surgery, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, P.R. China[*2]Hongqiao International Institute of Medicine, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, P.R. China
推荐引用方式(GB/T 7714)：
Feng Hai-Yi,Zhang Yunpeng,Liu Hai-Jun,et al.Characterization of an orthotopic gastric cancer mouse model with lymph node and organ metastases using bioluminescence imaging[J].ONCOLOGY LETTERS.2018,16(4):5179-5185.doi:10.3892/ol.2018.9313.
APA：
Feng, Hai-Yi,Zhang, Yunpeng,Liu, Hai-Jun,Dong, Xiao,Yang, Si-Cong...&Fang, Chao.(2018).Characterization of an orthotopic gastric cancer mouse model with lymph node and organ metastases using bioluminescence imaging.ONCOLOGY LETTERS,16,(4)
MLA：
Feng, Hai-Yi,et al."Characterization of an orthotopic gastric cancer mouse model with lymph node and organ metastases using bioluminescence imaging".ONCOLOGY LETTERS 16..4(2018):5179-5185
