机构：[1]Department of Hepatobiliary Surgery, Surgical Laboratory, Beijing Tong Ren Hospital, Capital Medical University, Beijing 100730, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Pathology, Capital Medical University, Beijing 100069, China
Background: Decoy receptor 3 (DcR3) is a protein with anti-apoptotic effect that belongs to the tumor necrosis factor receptor superfamily. DcR3 is highly expressed in a variety of malignant tumors including cholangiocarcinoma and its expression was found to be related to the clinical stage, the invasion, and the metastasis of the tumor. This in vitro study aimed to investigate the effect of downregulated expression of DcR3 on cell viability, cell apoptosis, and cell cycle in cholangiocarcinoma cell line TFK-1. Methods: Three different cell lines were cultured: human cholangiocarcinoma TFK-1, human biliary epithelial carcinoma HuCCT-1, and human cholangiocarcinoma RBE. The cholangiocarcinoma cell line with the highest expression of DcR3 was selected for further investigation. The expression of DcR3 was silenced/knocked down by transfection with DcR3-siRNA in the selected cell line. Various biological phenotype parameters such as cell viability, apoptosis, and cell cycle were observed. Results: The mRNA and protein levels of DcR3 were measured in the three cell lines, and TFK-1 was selected. After the treatment with DcR3-siRNA for 48 h, DcR3 mRNA and protein expression in the treatment group were 38.45% (P < 0.01) and 48.03% (P < 0.05) of that of the control, respectively. It was found that the cell viability decreased to 61.87% of the control group (P < 0.01) after the downregulation of DcR3 in cholangiocarcinoma cell line TFK-1 by transfection with DcR3-siRNA, while the percentage of apoptotic cells was 2.98 times as compared with the control group (P < 0.05). Compared with the control group the ratio of G(0)/G(1) increased, and the ratio of G(2)/M decreased in the treatment group. However, the differences were not statistically significant. Conclusions: The effect of DcR3 on the growth and apoptosis of cholangiocarcinoma has been demonstrated. DcR3 is not only a predictive marker for malignant tumor but it is also likely to be a potential target for cancer gene therapy. Further studies should focus on exploring the binding ligand of DcR3, the signaling pathway involved, and the molecular mechanism for the regulation of DcR3 expression in cholangiocarcinoma.
基金：
Basic
Clinical Cooperative Research Foundation of the Capital
Medical University (No. 15JL45 and 17JL65), the Beijing
Tongren Hospital Funds (No. TRYY‑KYJJ‑2015‑032),
the Capital Foundation of Medical Development (No.
shoufa2016‑2‑2053), and the Beijing Municipal
Administration of Hospitals Clinical Medicine Development
of Special Funding Support (No. ZYLX201612).
第一作者机构：[1]Department of Hepatobiliary Surgery, Surgical Laboratory, Beijing Tong Ren Hospital, Capital Medical University, Beijing 100730, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Hepatobiliary Surgery, Surgical Laboratory, Beijing Tong Ren Hospital, Capital Medical University, Beijing 100730, China[*1]Department of Hepatobiliary Surgery, Beijing Tong Ren Hospital, Capital Medical University, Beijing 100730, China
推荐引用方式(GB/T 7714)：
Xu Ying-Chen,Cui Jing,Zhang Li-Jun,et al.Anti-apoptosis Effect of Decoy Receptor 3 in Cholangiocarcinoma Cell Line TFK-1[J].CHINESE MEDICAL JOURNAL.2018,131(1):82-87.doi:10.4103/0366-6999.221271.
APA：
Xu, Ying-Chen,Cui, Jing,Zhang, Li-Jun,Zhang, Dong-Xin,Xing, Bing-Chen...&Li, Guang-Ming.(2018).Anti-apoptosis Effect of Decoy Receptor 3 in Cholangiocarcinoma Cell Line TFK-1.CHINESE MEDICAL JOURNAL,131,(1)
MLA：
Xu, Ying-Chen,et al."Anti-apoptosis Effect of Decoy Receptor 3 in Cholangiocarcinoma Cell Line TFK-1".CHINESE MEDICAL JOURNAL 131..1(2018):82-87
