机构：[1]Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing , China研究所眼科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Quinze-Vingts National Eye Center, Paris and Versailles Saint-Quentin-en-Yvelines University, Versailles,[3]INSERM U968, UPMC Université Paris-6, UMRS968, Institut de la Vision, CNRS, UMR7210,
Purpose: To characterize the phenotype of Reis-Bucklers corneal dystrophy (RBCD) using in vivo and ex vivo imaging techniques. Methods: Five RBCD patients with penetrating keratoplasty (PK) were enrolled. Before surgery, all patients underwent a complete ophthalmological examination including slitlamp biomicroscopy, in vivo confocal microscopy (IVCM) and anterior segment (AS) optical coherence tomography (OCT). After PK, corneal buttons were examined by light and transmission electron microscopy (TEM). Correlations between in vivo and ex vivo images were analyzed. Results: In all cases, irregular geographic-like subepithelial gray-white opacities were observed in the central and mid-peripheral cornea. AS-OCT images of the cornea of all patients revealed hyperreflective homogeneous and continuous deposits concentrated at the level of Bowman's layer and anterior stroma. Using IVCM, a highly reflective irregular amorphous material was observed from intermediate epithelial cells to the ante-nor stroma. Sparse deposits of highly reflective material were also detected in the posterior stroma. TEM showed in all specimens basal epithelial cells containing small vesicles with rodshaped dense material. Conclusions: IVCM and AS-OCT may be a useful adjunct to biomicroscopy for the diagnosis and management of RBCD. The correlations between the different in vivo and ex vivo imaging techniques emphasize the hypothesis of an epithelial origin for RBCD. (c) 2014 S. Karger AG, Basel
第一作者机构：[1]Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing , China
通讯作者：
通讯机构：[1]Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing , China[*1]Beijing Tongren Eye Center, Beijing Tongren Hospital Capital Medical University Beijing, 100005 (China)
推荐引用方式(GB/T 7714)：
Liang Qingfeng,Pan Zhiqiang,Sun Xuguang,et al.Reis-Bucklers Corneal Dystrophy: Reappraisal Using in vivo and ex vivo Imaging Techniques[J].OPHTHALMIC RESEARCH.2014,51(4):187-195.doi:10.1159/000358805.
APA：
Liang, Qingfeng,Pan, Zhiqiang,Sun, Xuguang,Baudouin, Christophe&Labbe, Antoine.(2014).Reis-Bucklers Corneal Dystrophy: Reappraisal Using in vivo and ex vivo Imaging Techniques.OPHTHALMIC RESEARCH,51,(4)
MLA：
Liang, Qingfeng,et al."Reis-Bucklers Corneal Dystrophy: Reappraisal Using in vivo and ex vivo Imaging Techniques".OPHTHALMIC RESEARCH 51..4(2014):187-195
