机构：[1]Institute of Medical Informatics, Chinese PLA General Hospital, Beijing 100853, China[2]The Ophthalmologic Center of Beijing University, Beijing 100083, China[3]Department of Ophthalmology, Chinese PLA General Hospital, Beijing 100853, China[4]Department of Pathology, Chinese PLA General Hospital, Beijing 100853, China[5]The Research Center of Pathophysiology, Chinese PLA General Hospital, Beijing 100853, China
To study optic nerve lesions, changes in blood flow to the optic nerve, and permeability of micro blood vessels and histopathology in diabetic rats.Male Wistar rats (n=20) were randomly divided into control and diabetic groups. The diabetic model was prepared by a single injection of streptozotocin (50mg/kg) into the caudal vein. Three months later, laser Doppler perfusion imaging was used to observe the changes in blood flow to the optic nerve. Each rat was injected with 15g/L Evans blue (5µL/g). The permeability of microvessels in diabetic optic nerves was measured by spectrophotometry. Optic nerves were observed by light and transmission electron microscopy.Diabetic rats had atrophic optic nerve fibers with neurite swelling, loss of myelin, and a greater-than-normal proliferation of astrocytes, occurring within 3 months of induction of diabetes. Blood flow to the optic nerve was lower in diabetic rats than in controls. Microvessel permeability in diabetic rats increased 2.03-fold compared to controls.Diabetic rats develop significant pathological changes in the optic nerve, reduced blood flow to the optic nerve and increase microvessel permeability.
基金：
Supported by National Natural Science
Foundation of China (No.60873067)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2009]版：
无
最新[2023]版：
大类|4 区医学
小类|4 区眼科学
第一作者：
第一作者机构：[1]Institute of Medical Informatics, Chinese PLA General Hospital, Beijing 100853, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhao Jun-Ping,Ma Zhi-Zhong,Song Chen,et al.Optic nerve lesions in diabetic rats: blood flow to the optic nerve, permeability of micro blood vessels and histopathology.[J].International Journal Of Ophthalmology.2010,3(4):291-4.doi:10.3980/j.issn.2222-3959.2010.04.03.
APA：
Zhao Jun-Ping,Ma Zhi-Zhong,Song Chen,Li Xiang-Hong,Li Yu-Zhen&Liu Yu-Ying.(2010).Optic nerve lesions in diabetic rats: blood flow to the optic nerve, permeability of micro blood vessels and histopathology..International Journal Of Ophthalmology,3,(4)
MLA：
Zhao Jun-Ping,et al."Optic nerve lesions in diabetic rats: blood flow to the optic nerve, permeability of micro blood vessels and histopathology.".International Journal Of Ophthalmology 3..4(2010):291-4
