The techniques available to locate the femoral tunnel during anterior cruciate ligament (ACL) reconstruction have notable limitations. To evaluate whether the femoral tunnel center could be located intraoperatively with a ruler, using the posterior apex of the deep cartilage (ADC) as a landmark. This retrospective case series included consecutive patients with ACL rupture who underwent arthroscopic single-bundle ACL reconstruction at the Department of Orthopedics, Beijing Tongren Hospital between January 2014 and May 2018. During surgery, the ADC of the femoral lateral condyle was used as a landmark to locate the femoral tunnel center with a ruler. Three-dimensional computed tomography (CT) was performed within 3 days after surgery to measure the femoral tunnel position by the quadrant method. Arthroscopy was performed 1 year after surgery to evaluate the intra-articular conditions. Lysholm and International Knee Documentation Committee (IKDC) scores were determined before and 1 year after surgery. The final analysis included 82 knees of 82 patients (age=31.7 +/- 6.1 years; 70 males). The femoral tunnel center was 26 +/- 1.5% in the deep-shallow (x-axis) direction and 31 +/- 3.1% in the high-low (y-axis) direction, close to the "ideal" values of 27 and 34%. Lysholm score increased significantly from 38.5 (33.5-47) before surgery to 89 (86-92) at 1 year after surgery ( p <0.001). IKDC score increased significantly from 42.5 (37-47) before surgery to 87 (83.75-90) after surgery ( p <0.001). Using the ADC as a landmark, the femoral tunnel position can be accurately selected using a ruler.