Ganoderma mushrooms have been widely used as functional food in China, Japan, and Korea. Ganoderma triterpenoids are deemed to be the main functional constituents. The structures of Ganoderma triterpenoids are complex but quite similar, which makes their analyses markedly limited. In this study, we developed a general 2D NMR method to differentiate Ganoderma triterpenoids, which classifies them into six types (A-F). Then, by the NMR-based isolation of A-F type triterpenoids from the fruiting bodies of G. resinaceum, four new compounds (1-4) and eight known compounds. (5-12) were obtained. Moreover, combined with spiking experiments in 1D and 2D NMR spectra, compounds 5, 7, and 8, which belong to triterpenoids of A and B types, were identified. At the end, to achieve a more extensive application for this NMR method, a qNMR method for the absolute quantification of 5, 7, and 8 in the gross triterpenoids from G. resinaceum was set up. The results showed that this NMR method is reliable for the NMR-guided isolation and quantification of triterpenoids in G. resinaceum.
基金：
National Natural Science Foundation of China [31872675, 21702209, 81172940]; Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China [P2010ZZ14]
基金编号： 81172940P2010ZZ14 2170220931872675
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|1 区农林科学
小类|1 区农业综合2 区应用化学2 区食品科技
最新[2025]版：
大类|1 区农林科学
小类|1 区农业综合2 区应用化学2 区食品科技
第一作者：
第一作者机构：[1]Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯机构：[3]Shanghai Jiao Tong Univ,Core Lab Med Ctr Clin Res,State Key Lab Med Gen,Shanghai Peoples Hosp 9,Dept Endocrinol,Sch Med,Shanghai 200011,Peoples R China
推荐引用方式(GB/T 7714)：
Huang Yanjie,Li Xian,Peng Xingrong,et al.NMR-based Structural Classification, Identification, and Quantification of Triterpenoids from Edible Mushroom Ganoderma resinaceum[J].JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.2020,68(9):2816-2825.doi:10.1021/acs.jafc.9b07791.
APA：
Huang, Yanjie,Li, Xian,Peng, Xingrong,Adegoke, Adelakun Tiwalade,Chen, Jianchao...&Qiu, Minghua.(2020).NMR-based Structural Classification, Identification, and Quantification of Triterpenoids from Edible Mushroom Ganoderma resinaceum.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,68,(9)
MLA：
Huang, Yanjie,et al."NMR-based Structural Classification, Identification, and Quantification of Triterpenoids from Edible Mushroom Ganoderma resinaceum".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 68..9(2020):2816-2825
农林科学