机构：[1]Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China,[2]Institute of Model Animal, Wuhan University, Wuhan, China,[3]Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, China,[4]Department of Urology, First Affiliated Hospital of Gannan Medical University, Ganzhou, China,[5]Gannan Innovation and Translational Medicine Research Institute, Gannan Medical University, Ganzhou, China,[6]College of Life Sciences, Wuhan University, Wuhan, China,[7]College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China,[8]School of Basic Medical Science, Wuhan University, Wuhan, China,[9]Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan, China,[10]Department of Pathophysiology, School of Basic Medical Sciences, Gannan Medical University, Ganzhou, China,[11]Department of Cardiology, Tongren Hospital of Wuhan University and Wuhan Third Hospital, Wuhan, China
Canine models are increasingly being used in metabolic studies due to their physiological similarity with humans. The present study aimed to identify changes in metabolic pathways and biomarkers with potential clinical utility in a canine model of obesity and metabolic disorders induced by a high-fat diet (HFD). Eighteen male beagles were included in this study, 9 of which were fed a HFD for 24 weeks, and the remaining 9 were fed normal chow (NC) during the same period. Plasma and urine samples were collected at weeks 12 and 24 for untargeted metabolomic analysis. Dogs fed a HFD showed a gradual body weight increase during the feeding period and had hyperlipidemia, increased leukocyte counts, and impaired insulin sensitivity at week 24. Plasma and urine metabonomics analysis displayed clear separations between the HFD-fed and NC-fed dogs. A total of 263 plasma metabolites varied between the two groups, including stearidonic acid, linolenic acid, carnitine, long-chain ceramide, 3-methylxanthine, and theophylline, which are mainly engaged in fatty acid metabolism, sphingolipid metabolism, and caffeine metabolism. A total of 132 urine metabolites related to HFD-induced obesity and metabolic disorders were identified, including 3-methylxanthine, theophylline, pyridoxal 5'-phosphate, and harmine, which participate in pathways such as caffeine metabolism and vitamin digestion and absorption. Eight metabolites with increased abundance (e.g., 3-methylxanthine, theophylline, and harmine) and 4 metabolites with decreased abundance (e.g., trigonelline) in both the plasma and urine of the HFD-fed dogs were identified. In conclusion, the metabolomic analysis revealed molecular events underlying a canine HFD model and identified several metabolites as potential targets for the prevention and treatment of obesity-related metabolic disorders.
基金：
National Science Foundation of China [81770053, 81970364, 82000538, 82100086]; Hubei Province Innovation Platform Construction Project [20204201117303072238]; Henan Charity General Federation-Hepatobiliary Foundation of Henan Charity General Federation [GDXZ2019010]; Wuhan Science and Technology Planning Project [2020021105012439]
第一作者机构：[1]Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China,[2]Institute of Model Animal, Wuhan University, Wuhan, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China,[2]Institute of Model Animal, Wuhan University, Wuhan, China,[8]School of Basic Medical Science, Wuhan University, Wuhan, China,
推荐引用方式(GB/T 7714)：
Qu Weiyi,Chen Ze,Hu Xing,et al.Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model[J].FRONTIERS IN ENDOCRINOLOGY.2022,13:doi:10.3389/fendo.2022.849060.
APA：
Qu, Weiyi,Chen, Ze,Hu, Xing,Zou, Toujun,Huang, Yongping...&Li, Hongliang.(2022).Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model.FRONTIERS IN ENDOCRINOLOGY,13,
MLA：
Qu, Weiyi,et al."Profound Perturbation in the Metabolome of a Canine Obesity and Metabolic Disorder Model".FRONTIERS IN ENDOCRINOLOGY 13.(2022)
