Accumulating evidence has indicated the significant roles of long noncoding RNAs (lncRNAs) in the pathophysiology of diabetic nephropathy (DN). LncRNA nuclear enriched abundant transcript 1 (NEAT1) has been reported to exert a key role in the progression of several diseases including diabetes. However, the role of NEAT1 in the regulation of DP progression remains barely known. Therefore, our study aimed to investigate the role of NEAT1 in a streptozotocin-induced diabetes model (DM) of rats and glucose-induced mouse mesangial cell models. Currently, we found that NEAT1 was greatly upregulated in DM rats and glucose-induced mice mesangial cells, in which a high activation of Akt/mTOR signaling was also observed. Then, it was shown that knockdown of NETA1 was able to reduce renal injury in DM rats obviously. In addition, cell counting kit-8 assay and 5-ethynyl-2-deoxyuridine assay were carried out and we observed downregulation of NEAT1 significantly inhibited mesangial cell proliferation. Meanwhile, extracellular matrix proteins and messenger RNA (transforming growth factor 1, fibronectin, and collagen IV) expression was dramatically restrained by silencing of NEAT1 in the high glucose-induced mesangial cells. Finally, knockdown of NEAT1 greatly reduced the expression of the phosphorylation of Akt and mammalian target of rapamycin (mTOR) in vitro. These findings revealed that the decrease of NEAT1 repressed the proliferation and fibrosis in DN via activating the Akt/mTOR signaling pathway, which might represent a novel pathological mechanism of DN progression.
基金：
Changning District Committee of Science and Technology [CZKW2016Z03]
第一作者机构：[1]Shanghai Jiao Tong Univ, Shanghai Tongren Hosp, Sch Med, Endocrinol Dept, Shanghai, Peoples R China[*1]Endocrinology Department, School of Medicine, Shanghai Tongren Hospital, Affiliated to Shanghai Jiao Tong University, Shanghai, China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Shanghai Tongren Hosp, Sch Med, Endocrinol Dept, Shanghai, Peoples R China[*1]Endocrinology Department, School of Medicine, Shanghai Tongren Hospital, Affiliated to Shanghai Jiao Tong University, Shanghai, China
推荐引用方式(GB/T 7714)：
Huang Shan,Xu Yong,Ge Xiaoxu,et al.Long noncoding RNA NEAT1 accelerates the proliferation and fibrosis in diabetic nephropathy through activating Akt/mTOR signaling pathway[J].JOURNAL OF CELLULAR PHYSIOLOGY.2019,234(7):11200-11207.doi:10.1002/jcp.27770.
APA：
Huang, Shan,Xu, Yong,Ge, Xiaoxu,Xu, Bojin,Peng, Wenfang...&Xia, Lili.(2019).Long noncoding RNA NEAT1 accelerates the proliferation and fibrosis in diabetic nephropathy through activating Akt/mTOR signaling pathway.JOURNAL OF CELLULAR PHYSIOLOGY,234,(7)
MLA：
Huang, Shan,et al."Long noncoding RNA NEAT1 accelerates the proliferation and fibrosis in diabetic nephropathy through activating Akt/mTOR signaling pathway".JOURNAL OF CELLULAR PHYSIOLOGY 234..7(2019):11200-11207
