机构：[1]Beijing Tongren Eye Center, Capital Medical University Affiliated Beijing Tongren Hospital, Vision Science Laboratory, School of Ophthalmology, Beijing 100730, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Neurobiology and Beijing Institute for Neuroscience, Capital Medical University, #10 You An Men Wai Xi Tou Tiao, Beijing 100069, China
Evidence indicates that conventional protein kinase C (cPKC) plays a pivotal role in the development of retinal ischemic preconditioning (IPC). In this study, the effect of high intraocular pressure (IOP)-induced retinal IPC on cPKC isoform-specific membrane translocation and protein expression were observed. We found that cPKC gamma membrane translocation increased significantly at the early stage (20 min-1 h), while the protein expression levels of cPKC alpha and gamma were markedly elevated in the delayed retinal IPC (12-168 h) of rats. The increased protein expressions of cPKC alpha at 72 h and cPKC gamma at 24 h after IPC were further confirmed by immunofluorescence staining. In addition, we found that cPKC gamma co-localized with retinal ganglion cell (RGC)-specific marker, neurofilaments heavy chain (NF-H) by using double immunofluorescence labeling. These results suggest that increased cPKC gamma membrane translocation and up-regulated protein expressions of cPKC alpha and gamma are involved in the development of high IOP-induced rat retinal IPC. (c) 2008 Elsevier Ltd. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30571991, 30670782, 30871219]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7043067, 5072008]; Key Scientific Developing Program of Beijing Municipal Commission of Education [KZ200810025012]; Institutions of Higher Learning Under the jurisdiction of Beijing Municipality (Li J) and China 973 ProgramNational Basic Research Program of China [2006CB504100]
第一作者机构：[1]Beijing Tongren Eye Center, Capital Medical University Affiliated Beijing Tongren Hospital, Vision Science Laboratory, School of Ophthalmology, Beijing 100730, China
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推荐引用方式(GB/T 7714)：
Ding Jingwen,Ding Ning,Wang Ningli,et al.Determination of conventional protein kinase C isoforms involved in high intraocular pressure-induced retinal ischemic preconditioning of rats[J].VISION RESEARCH.2009,49(3):315-321.doi:10.1016/j.visres.2008.10.018.
APA：
Ding, Jingwen,Ding, Ning,Wang, Ningli,Lu, Qingjun,Lu, Ningning...&Li, Junfa.(2009).Determination of conventional protein kinase C isoforms involved in high intraocular pressure-induced retinal ischemic preconditioning of rats.VISION RESEARCH,49,(3)
MLA：
Ding, Jingwen,et al."Determination of conventional protein kinase C isoforms involved in high intraocular pressure-induced retinal ischemic preconditioning of rats".VISION RESEARCH 49..3(2009):315-321
