Basal cell carcinoma is driven by the aberrant activation of hedgehog signaling. DEAD (Asp-Glu-Ala-Asp) box protein 5 is frequently overexpressed in human cancer cells and associated with the tumor growth and invasion. The purpose of this study was to investigate the role of DEAD (Asp-Glu-Ala-Asp) box protein 5 in the growth, migration, and invasion of basal cell carcinoma. The role of DEAD (Asp-Glu-Ala-Asp) box protein 5 was detected by quantitative real-time polymerase chain reaction, Western blot, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assay in basal cell carcinoma cells. The associations between JAK2/STAT3 pathway and DEAD (Asp-Glu-Ala-Asp) box protein 5 were analyzed in basal cell carcinoma cells. Results showed that DEAD (Asp-Glu-Ala-Asp) box protein 5 is overexpressed in basal cell carcinoma cells. DEAD (Asp-Glu-Ala-Asp) box protein 5 knockdown inhibited the migration and invasion of basal cell carcinoma cells. DEAD (Asp-Glu-Ala-Asp) box protein 5 knockdown increased the apoptosis of basal cell carcinoma cells induced by tunicamycin. Results found that DEAD (Asp-Glu-Ala-Asp) box protein 5 knockdown increased JAK2 and STAT3 expression in basal cell carcinoma cells. JAK2 inhibitor decreased STAT3 expression and abolished the inhibitory effects of DEAD (Asp-Glu-Ala-Asp) box protein 5 silencing on migration and invasion in basal cell carcinoma cells. In conclusion, these results indicate that DEAD (Asp-Glu-Ala-Asp) box protein 5 is a potential target for inhibiting basal cell carcinoma cells growth, migration, and invasion by downregulating JAK2/STAT3 pathway.
基金：
Shanghai Tongren Hospital [TRYJ201508]; Changning district health and family planning commission [20164Y005]
第一作者机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Dematol, Sch Med, 1111 Xianxia Rd, Shanghai 200336, Peoples R China[*1]Department of Dematology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No 1111, Xianxia Road, 200336, Shanghai, China.
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Dematol, Sch Med, 1111 Xianxia Rd, Shanghai 200336, Peoples R China[*1]Department of Dematology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, No 1111, Xianxia Road, 200336, Shanghai, China.
推荐引用方式(GB/T 7714)：
Quan Zhe,Zhang Bei-bei,Yin Fang,et al.DDX5 Silencing Suppresses the Migration of Basal cell Carcinoma Cells by Downregulating JAK2/STAT3 Pathway[J].TECHNOLOGY IN CANCER RESEARCH & TREATMENT.2019,18:doi:10.1177/1533033819892258.
APA：
Quan, Zhe,Zhang, Bei-bei,Yin, Fang,Du, Jiru,Zhi, Yuan-ting...&Song, Ningjing.(2019).DDX5 Silencing Suppresses the Migration of Basal cell Carcinoma Cells by Downregulating JAK2/STAT3 Pathway.TECHNOLOGY IN CANCER RESEARCH & TREATMENT,18,
MLA：
Quan, Zhe,et al."DDX5 Silencing Suppresses the Migration of Basal cell Carcinoma Cells by Downregulating JAK2/STAT3 Pathway".TECHNOLOGY IN CANCER RESEARCH & TREATMENT 18.(2019)
