机构：[1]Department of Orthopedics, Tongren Hospital, School of Medicine, Shanghai JiaoTong University, 1111 Xianxia Road Shanghai, Shanghai 200336, P.R. China
Background To clarify the role of apolipoprotein D (Apod) in alleviating glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells (MSCs) via the PI3K/Akt pathway, thus influencing the progression of osteoporosis (OP). Methods Osteogenesis in MSCs was induced by dexamethasone (DEX) stimulation. Dynamic expressions of Apod in MSCs undergoing osteogenesis for different time points were determined by qRT-PCR. Relative levels of osteogenesis-associated genes, including ALP, RUNX2, and Osterix, in DEX-induced MSCs overexpressing Apod or not were examined. Moreover, the protein level of RUNX2, ALP, and Osterix; ALP activity; and mineralization ability influenced by Apod in osteogenic MSCs were assessed. At last, the potential influences of Apod on the PI3K/Akt pathway were identified through detecting the expression levels of PI3K and Akt in MSCs by Western blot. Results Apod was time-dependently upregulated in MSCs undergoing osteogenesis. DEX induction downregulated ALP, RUNX2, and Osterix and attenuated ALP activity and mineralization ability in MSCs undergoing osteogenesis, which were partially reversed by overexpression of Apod. In addition, Apod overexpression upregulated the reduced levels of PI3K and Akt in DEX-induced MSCs. Conclusion Apod alleviates glucocorticoid-induced osteogenesis suppression in MSCs via the PI3K/Akt pathway, thus protecting the progression of OP.
基金：
This work was supported by the National Natural Science Foundation of
China (No. 81903039), the Sponsored by Shanghai Sailing Program (No.
19YF1444500), the Excellent young medical talents training plan of the
Shanghai Health Planning Commission (No. 2018YQ46), the Changning
District Committee of Science and Technology (No. CNKW2017Y07), the
Changning District Health and Family Planning Commission (No. 20164Y003,
20194Y008), and the Sixth People’s Hospital of Shanghai Medical Group
Projects (No. ly201802, ly201903).
第一作者机构：[1]Department of Orthopedics, Tongren Hospital, School of Medicine, Shanghai JiaoTong University, 1111 Xianxia Road Shanghai, Shanghai 200336, P.R. China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Yu Rong-Hua,Zhang Xiang-yang,Xu Wei,et al.Apolipoprotein D alleviates glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells via the PI3K/Akt pathway[J].JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH.2020,15(1):doi:10.1186/s13018-020-01824-1.
APA：
Yu, Rong-Hua,Zhang, Xiang-yang,Xu, Wei,Li, Zhi-kun&Zhu, Xiao-dong.(2020).Apolipoprotein D alleviates glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells via the PI3K/Akt pathway.JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH,15,(1)
MLA：
Yu, Rong-Hua,et al."Apolipoprotein D alleviates glucocorticoid-induced osteogenesis suppression in bone marrow mesenchymal stem cells via the PI3K/Akt pathway".JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH 15..1(2020)
